沙巴体育 Focus:

Dr. Uttara Chakraborty is an Assistant Professor in the 沙巴体育 Institute of Regenerative Medicine since 2019. She has established the Stem cell Cytometry and Imaging lab at MIRM, which is committed towards interdisciplinary research where she uses stem cells as ideal research models integrating with the concepts of computational biology, molecular mycology and clinical research to understand the potential of these cells in immunotherapy. She is also active in developing protocols for developing shared resource laboratory of Flow cytometry, Cell sorting and Cell imaging in association with the 沙巴体育 Society of Advancement of Cytometry (ISAC), a dedicated 沙巴体育 Association of cell cytometry. Her lab is working on 4 important areas of research:

A. In search of exploring mesenchymal stem cell morphometric and immuno-heterogeneity using the imaging cytometry approach

B. Towards developing a single cell image-based tool to understand the correlation of plots to pixels

C. Translate a novel technology to develop an efficient, cost-effective diagnostic tool for sensitive and concurrent phenotypic/genotypic classification of Acute Lymphoblastic Leukemia (ALL) malignancies in Indian patients

D. Establishment of a new in vitro infection model using mesenchymal stem cells to understand the role of MSCs in clearance of bacterial and fungal pathogens of the body.

Publications:

1. Kewal Asosingh, Alice Bayiyana, Michele C. Black, Uttara Chakraborty, Michael J. Clemente, Amy C. Graham, Michael D. Gregory, Karen G. Hogg 8, Gert Van Isterdael, ChunChun Liu, Lola Martínez, Charlotte C. Petersen, Ziv Porat, Kylie M. Price, Laura B Prickett, Aja M. Rieger, Caroline E. Roe, Erica Smit. (2024) Best Practices for User Consultation in Flow Cytometry Shared Resource Laboratories. Cytometry A Q1 journal

2. Risani Mukhopadhyay, Pulkit Chandel, Keerthana Prasad, Uttara Chakraborty. (2024) Machine learning aided single cell image analysis improves understanding of morphometric heterogeneity of human mesenchymal stem cells. Methods. Mar 13; 225:62-73. doi: 10.1016/j.ymeth.2024.03.005. Epub ahead of print. PMID: 38490594. Q1 journal

3. Caroline E. Roel, Vera A. Tang, Jessica B. Back, Anna C. Belkina, Claudia Bispo, Alexis Conway, Uttara Chakraborty, Kathleen T. Daniels, Gelo de la Cruz, Laura Ferrer-Font, Andrew Filby, David M. Gravano, Michael D. Gregory, Christopher Hall, Christian Kukat, André Mozes, Diana Ordo?ez-Rueda, Eva Orlowski- Oliver, Isabella Pesce, Ziv Porat, Nicole J. Poulton, Kristen M. Reifel, Aja M. Rieger, Rachael T.C. Sheridan, Gert Van Isterdael & Rachael V. Walker. (2023) Guidelines for Establishing a Cytometry Laboratory. Cytometry A. DOI: 10.1002/cyto.a.24807. Q1 journal

4. Ayona Gupta, Safia.K. Shaik, Lakshmi Balasubramanian, Uttara Chakraborty (2023) MSCProfiler: An image processing workflow to investigate Mesenchymal Stem Cell heterogeneity using imaging flow cytometry data. Biotechniques. DOI:10.2144/btn-2023-0048 Q2 journal

*Outcome of the Luminex Corp. (now Cytek Biosciences) contract with MIRM for the Amnis Imagestream Imaging Cytometer.

5. Ayona Gupta, Risani Mukhopadhyay, Himanshi Khandelwal, Narendra Nala, Uttara Chakraborty (2023). Single cell sorting of immunophenotyped mesenchymal stem cells from human exfoliated deciduous teeth. J. of Visualized Experiments. DOI:10.3791/65723. Q3 journal

*Supported by the Intramural MAHE grant 2022-2025.

6. Aishwarya Nagarajan; Nasera Rizwana; Michelle Abraham; Mahima Bhat; Aakanksha Vetekar; Goutam Thakur; Uttara Chakraborty; Vipul Agarwal; Manasa Nune. (2023). Polycaprolactone/graphene oxide/acellular matrix nanofibrous scaffolds with antioxidant and promyelinating features for the treatment of peripheral demyelinating diseases. Journal of Materials Science: Materials in Medicine, 34:49. DOI: 10.1007/s10856-023-06750-2 Q1 journal

7. Mukhopadhyay, R., Varshitha, D., Telford, W. G., Sanders, C. K., & Chakraborty, U. (2023). Mammalian chromosome analysis and sorting by flow cytometry. Current Protocols, 3, e785. DOI: 10.1002/cpz1.78 Q1 journal

8. Gravano DM, Chakraborty U, Pesce I, Thomson M. (2021) Solutions for Shared Resource Lab Remote Quality Control and Instrument Troubleshooting during a Pandemic. Cytometry Part A. Jan; 99(1):51-59. doi: 10.1002/cyto.a.24266. Q1 journal

9. Chakraborty, U. 2019. Mycotechnology: Advancement with the Application of Modern Technique, In: Fundamentals of Biodiversity, Biotechnology and Bioinformatics, Ed. Samit Ray, New Central Book Agency, Kolkata, New Delhi (accepted). Book Chapter

10. Chakraborty U, Mohamed A, Kakade P, Mugasimangalam RC, Sadhale PP, Sanyal K (2013) A stable hybrid containing haploid genomes of two obligate diploid Candida species. Euk Cell 12: 1061-1071

11. Chakraborty U, Sikdar SR (2010) Intergeneric protoplast fusion between Calocybe indica (milky mushroom) and Pleurotus florida aids in the qualitative and quantitative improvement of sporophore of the milky mushroom. World J Microbiol Biotechnol 26: 213–225 (DOI 10.1007/s11274-009-0162-8)

12. Chakraborty U, Sikdar SR (2008) Production and characterization of somatic hybrids raised through protoplast fusion between edible mushroom strains Volvariella volvacea and Pleurotus florida. World J Microbiol Biotechnol 24:1481–1492 (DOI 10.1007/s11274-007-9630-1)

13. Chakraborty U, Sikdar SR (2007) Breaking the barriers of conventional mushroom breeding- Protoplast Fusion can bring revolution in the Mushworld. Mushroom Biol and Biotechnol 89- 113

Scopus link https://researcher.manipal.edu/en/persons/uttara-chakraborty